The active site of yeast phosphoglycerate kinase.

نویسندگان

  • H C Watson
  • T N Bryant
  • N P Walker
  • P J Shaw
  • P L Wendell
چکیده

Eby, D. & Kirtley, M. E. (1971) Biochemistry 10,2677-2682 Fuller-Noel, J. K. & Schumaker, V. W. (1972) J. Mol. Biol. 68,523-532 Gennis, L. S. (1976) Proc. Natl. Acad. Sci. U.S.A. 73, 3928-3932 Harrigan, P. J. & Trenthami, D. R. (1971) Biochem. J . 124, 573-580 Hams, J. I. & Polgar, L. (1965) J. Mol. Biol. 14, 630-633 Harris, J. I. & Waters, M. (1976) Enzymes 3rd Ed. 23, 1-50 Kirschner, K. (1971) J. Mol. Biol. 5 8 , 5 1 4 8 Kirschner, K., Gallego, E., Schuster, I. & Goodall, D. (1971) J. Mol. Biol. 58, 29-50 Koshland, D. E., Jr., Nerneithy, G. & Filmer, D. (1966) Biochemistry 5, 365-385 Levitzki, A. (1974) J. Mol. Biol. 90, 451458 MacQuarrie, R. & Bernharcl, S. A. (1971) J. Mol. Biol. 55, 181-192 Malhotra, 0. P. & Bernhard, S. A. (1968) J. Biol. Chem. 243, 1243-1252 Malhotra, 0. P. & Bernharcl, S. A. (1973) Proc. Natl. Acad. Sci. U.S.A. 70,2077-2081 Monod, J., Wyman, J. & Changeux, J. P. (1965) J. Mol. Biol. 12, 88-118 Moras, D., Olsen, K. W., Sabesan, M. N.,Buehner, M., Ford, G. C. & R O S S ~ M , . G. (1975)

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Arrangement of substrates at the active site of yeast phosphoglycerate kinase. Effect of sulfate ion.

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Phosphorylation influences the binding of the yeast RAP1 protein to the upstream activating sequence of the PGK gene.

Yeast repressor activator protein 1 (RAP1) binds in vitro to specific DNA sequences that are found in diverse genetic elements. Expression of the yeast phosphoglycerate kinase gene (PGK) requires the binding of RAP1 to the activator core sequence within the upstream activating sequence (UAS) of PGK. A DNA fragment Z+ which contains the activator core sequence of the PGK(UAS) has been shown to b...

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Chemical modification of yeast 3-phosphoglycerate kinase.

Sulfhydryl reagents, as well as mild hydrogen peroxide oxidation, do not inhibit the activity of yeast phosphoglycerate kinase, indicating that the single thiol group and 3 methionine residues present in the enzyme are not essential for activity. Nitration of phosphoglycerate kinase by tetranitromethane inhibits the enzyme by reaction with a single tyrosine residue. Substrates provide partial p...

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A cell-free extract from yeast cells for studying mRNA turnover.

We have isolated a cell-free extract from yeast cells that reproduces the differences observed in vivo in the rate of turnover of individual yeast mRNAs. Detailed analysis of the degradation of yeast phosphoglycerate kinase (PGK) mRNA in this system demonstrated that both natural and synthetically prepared PGK transcripts are degraded by the same pathway previously established by us in vivo, co...

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عنوان ژورنال:
  • Biochemical Society transactions

دوره 5 3  شماره 

صفحات  -

تاریخ انتشار 1977